Cancer Metastasis & Adhesion Group

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Vasiliki Gkretsi

Group Leader
Associate Professor, Cellular & Molecular Pathology Biomedical Sciences Program Coordinator
Department of Life Sciences
European University Cyprus

Short Bio:

Dr. Gkretsi is an Associate Professor of Cellular and Molecular Pathology at the Department of Life Sciences of the European University Cyprus and currently serves as the coordinator of the Biomedical Sciences Program. She received her Diploma in Biology from the National and Kapodistrian University of Athens, Greece in 2001 and her Ph.D in Cellular and Molecular Pathology from the University of Pittsburgh Medical School, Pittsburgh, USA in 2006. Following completion of her Ph.D, she worked as post-doctoral fellow under the Marie Curie ITN program at the BSRC Al. Fleming in Athens, Greece. In 2009 she was elected Researcher D at the Center for Research & Technology-Thessaly (CE.RE.TE.TH), Larissa, Greece and was promoted to Researcher C (Research Assistant Professor) in 2012 in the same institution (now CE.R.T.H-Centre for Research & Technology-Hellas). She acquired funding from Greek EU funded programs and was the recipient of the prestigious European Association for the Study of the Liver (EASL) Sheila Sherlock fellowship award 2012. During 2010-2014, she served as a visiting lecturer at the University of Thessaly Medical School in Greece while from 2014-2017 she worked as a senior scientist in the Cancer Biophysics Lab of the University of Cyprus. In total, she has published 45 papers in peer reviewed international scientific journals and her work has received over 1100 citations (1166 by Scopus and 1692 by Google scholar) with an h index of 18 (Scopus) and 22 (Google scholar), respectively. She has presented her work in 11 poster and 16 oral presentations in international scientific conferences while she also serves as reviewer in 26 scientific journals and as an Editorial Board member in four (4). Dr. Gkretsi is currently the head of the “Cancer Metastasis and Adhesion“ research group of the newly founded Basic and Translational Cancer Research Center . Her current research is focusing on the study of the role of extracellular matrix (ECM), integrin-proximal protein complexes, cell adhesion and the cytoskeleton in basic cellular processes related to cancer progression and metastasis. In an attempt to better approximate the real tumor setting and microenvironment both two-dimensional (2D) and 3D cell culture models are used. 3D models include culturing cancer cells or tumor spheroids in collagen gels. The ultimate goal is the determination of the molecular mechanism driving cancer cell metastasis in relation to ECM and the identification of novel potential metastasis biomarkers and/or therapeutic targets. 

Research focus:

As cancer-associated mortality is mainly due to cancer cell metastasis and not due to the primary tumor itself, our research is focusing on understanding the process of metastasis at the cellular and molecular level, identifying the key molecular players and determining potential metastasis-biomarkers and therapeutic targets. In fact, we are especially interested in the involvement of integrin-proximal protein complexes, extracellular matrix and actin cytoskeleton in basic cellular processes implicated in cancer cell metastasis, such as proliferation, apoptosis, adhesion, migration and invasion of surrounding tissues. Additionally, understanding the connection between inflammation and the tumor microenvironment and how this can affect cancer cell metastasis is also within our research interests. To that regard, a combination of cellular and molecular techniques is being utilized using both two dimensional (2D) and 3D in vitro model systems. 3D models include culturing cancer cells in 3D collagen gels and tumor spheroid development and embedding in collagen gels in an attempt to mimic in vitro, the tumor growth that takes place at the real tumor setting. The ultimate goal is the determination of the molecular mechanism driving cancer cell metastasis in relation to extracellular matrix and the identification of novel potential metastasis biomarkers or therapeutic targets.

Current Group Members

Louiza Kozaki_BSc_BMS

Louiza Kozaki

BSc
BSc student,
Her main research interests include the study of the role of Growth Differentiation Factor -15 (GDF15) in the metastatic properties of colon cancer cells.
Nektaria Chrusanthou_BSc_BMS

Nektaria Chrysanthou

BSc
BSc student,
Her main research interests include the study of the role of Ras Suppressor-1 (RSU-1) in the metastatic properties of colon cancer cells.
Andria Kotsoni_BSc_BMS

Andria Kotsoni

BSc
BSc student,
Her main research interests include transformation of bacteria with Green Fluorescent Protein (GFP)-tagged RSU1 and overexpression of GFP-RSU1 by transfection into MCF-7 breast cancer cells.
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Constantina Theori

BSc
BSc student,
Her main interests include the study of the involvement of Long Non-coding RNAs in breast cancer metastasis with regard to function and clinical value.
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Leonidas Christodoulou

BSc
BSc student,
His main interests include the study of liver regeneration and the factors that terminate it.
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Constantinos Constantinou

BSc
BSc student,
MSc Thesis Title: His main interests include the study of the role of Parvins in cancer cell metastasis.
John Jbeily_MSc_Cancer Biology

John Jbeily

BSc
MSc student,
His main research interests include transformation of bacteria with Green Fluorescent Protein (GFP)-tagged RSU1 and overexpression of GFP-RSU1 by transfection into H4 neuroglioma cells.
Mokios Kyrillou_MSc_Cancer Biology

Mokios Kyrillou

BSc
MSc student,
His main research interests include the study of the role of RSU1 on ibuprofen-treated invasive breast cancer cells with regard to proliferation, migration and invasion with and without chemotherapy.
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Stephania Adamopolou

BSc
BSc student,
Her main interests include the study of the in vitro work on the effect of Non steroidal anti-inflammatory drugs in cancer.
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Kyriaki Komninou

BSc
BSc student,
Her main interests include the study of the in vitro work on the effect of Non steroidal anti-inflammatory drugs in cancer.
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Anastasia Kamanets

BSc
BSc student,
Her main interests include the study of the effect of Non steroidal anti-inflammatory drugs in cancer patients.
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Panayiotis Lipsos

BSc
BSc student,
MSc Thesis Title: His main research interests include evaluation of anticancer and antioxidant properties of essential oils.

Past Group Members

Christou Christiana

Christiana Christou

BSc
MSc student,
MSc Thesis Title: Investigation of the effect of RSU1 silencing on basic apoptotic signaling pathways in breast cancer and glioblastoma cells.
Eleana Demetriou

Eleana Demetriou

BSc
MSc student,
MSc Thesis Title: Study of the role of RSU1 on ibuprofen-treated breast cancer cells: effects on proliferation, migration and invasion.
Constantina Solonos-02

Constantina Solonos

BSc
MSc student,
MSc Thesis Title: Investigation of gene expression changes in breast cancer cells treated with aspirin.
Eleni Stylianou

Eleni Stylianou

BSc
MSc student,
MSc Thesis Title: Study of the role of RSU1 on aspirin-treated breast cancer cells: effects on proliferation, migration and invasion.

Lefteris Lakerides

MSc, (2021)
MSc Thesis Title: Investigation of the effect of estrogens and their metabolite 2 methoxy estradiol (2ME) on breast cancer cell gene expression. Current position: Biologist at NIPD genetics, Cyprus.

Gioumie Christina Koskina

MSc, (2021)
MSc Thesis Title: Study of the role of Ras Suppressor-1 (RSU-1) in cancer cell metastasis; comparison between colon cancer cell lines with normal and mutant Ras oncogene.

Katerina Kyriacou

MSc, (2021)
MSc Thesis Title: Study of the role of Growth Differentiation Factor-15 in glioma cell metastasis. Current position: Biologist at NIPD genetics, Cyprus.

Christiana Christou

BSc (2020)
Study of the effect of Ras Suppresor 1 (RSU1) silencing on apoptotic gene expression in breast cancer cells. Current position: MSc student at EUC.

Anna Spanopoulou

(2019)
The role of Growth Differentiation Factor-15 (GDF-15) in cancer cell metastasis. Current position: MSc student at the University of Athens.

Publication from her BSc Thesis:
Spanopoulou A., and Gkretsi V*. (2020) Growth Differentiation Factor-15 (GDF-15) in cancer cell metastasis: from the cells to the patients. Clinical & Experimental Metastasis 37(4):451-464. doi: 10.1007/s10585-020-10041-3

Katerina Tsirtsaki

BSc (2020)
Role of focal adhesion protein “Integrin Linked Kinase (ILK)” in breast cancer pathogenesis. Current position: MSc student.

Publication from her BSc Thesis:
Tsirtsaki K., and Gkretsi V. (2020) The focal adhesion protein Integrin Linked Kinase (ILK) as an important player in breast cancer pathogenesis. Cell Adhesion and Migration 14(1):204-213. doi: 10.1080/19336918.2020.1829263

Georgiou Vasiliki

(2018)
BSc Thesis Title: The role of Fibroblast Growth Factor in Glioma Pathogenesis. Current position: MSc student at Cyprus Institute of Neurology & Genetics.

Publication from her BSc Thesis:
Georgiou V., and Gkretsi V. (2018) The role of Fibroblast Growth Factors and their receptors in gliomas; mutations involved Rev Neurosci, 2018 Oct 31, doi: 10.1515/revneuro-2018-0051.

Research Grants

Completed

European Society for the Study of the Liver, 2012 EASL Sheila Sherlock award (October 2012-November 2014). Role: Principal investigator. Title: «The role of integrin-proximal protein complexes during hepatocellular carcinoma metastasis».

General Secretariat for Research and Technology (GSRT), Action: NSFR (Protocol number: 12-11/09 #705, 07/01/2011-07/01/2014). Role: Principal investigator. Title: «Osteoarthritis: complete systematic analysis for targeted biologic therapy».

Selected Publications

Book chapters

Full list of publications

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